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This behavioral assay happened in two trials. In the primary demo, Every single animal was paired that has a littermate within the house cage of the subject animal. In the next demo, Each and every animal was paired by using a novel mouse with which they'd no prior Call. Except for animal pairing requirements, both of those phases have been carried out utilizing the exact methodology. Two mice were being positioned within an vacant, plastic, sq. (25 × twenty five cm) arena and permitted to check out or interact without the need of interference. This conversation was recorded by using an overhead camcorder and scored in authentic-time by TopScan monitoring application (CleverSys). One particular mouse in each paired trial was marked with a small dot of white-out Resolution streaked on its fur to aid the TopScan software package in differentiating The 2 mice over the trial.
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To ascertain whether the Pro32Pro33 mutation influences the αvβ3 receptor–SERT conversation in midbrain synapses, we done coimmunoprecipitation experiments.
These data point out that various signaling pathways downstream from PAR4 activation, beyond Src, modify platelet attachment while in the context from the Pro32Pro33 mutation.
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All mice had been group housed in temperature- and humidity-controlled ailments underneath a 12-hour gentle/darkish cycle with foodstuff and drinking water out there advert libitum. All studies have been done in accordance with humane suggestions set up because of the Vanderbilt Institutional Animal Care and Use Committee below pro33 demo an authorized protocol (M/11/065).
locus, replacing the endogenous Ser32Gln33. Pro32Pro33 mice have altered adhesion and enhanced velocity in aggregation, leading to a proaggregatory phenotype noticed in tail bleeding and nonfatal thromboembolism.